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KMID : 1059520120560050577
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Journal of the Korean Chemical Society
2012 Volume.56 No. 5 p.577 ~ p.582
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Quinine Assay with Home-Built UV-LED Fluorometer: Quantitative Analysis, Photo-Bleaching, Fluorescence Quenching, and Urine Analysis
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Cheon Tae-Min
Cheong Byeong-Seo
Cho Han-Gook
Kim Jin-Hee
Kim Kyoug-Soo
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Abstract
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Quinine quantitative analysis, photo-bleaching, fluorescence quenching, and urine analysis have been performed by means of a UV-LED fluorometer, which can be easily built and used in a high-school laboratory. The quinine detection range is estimated to be 0.05-80 ppm, enough for many classroom luminescence experiments. The quinine content in commercial tonic water is determined from the calibration curve, and UV photo-bleaching of this anti-malarial drug is demonstrated with clear wavelength dependence. Halide quenching of quinine fluorescence is also observed and the increase in quenching efficiency in the order of Cl-, Br-, and I- is evident. Urine analyses for the student volunteers have been carried out and the results clearly reveal excretion of the ingested quinine. The student participants are exuberant throughout the course of this study and sense the practices resourceful.
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KEYWORD
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Quinine, Fluorescence, UV-LED, Fluorescence quenching, Urine analysis
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